Normal- and Reversed-Phase Behaviour of 16,17-Secoestrone Derivatives on Cyanopropyl-Bonded Silica Gel

The retention behaviour of a number of 16,17-secoestrone derivatives has been studied by LC and HPTLC on a polar cyanopropyl-bonded stationary phase using non-aqueous and aqueous-organic mobile phases. The retention behaviour has been discussed in terms of nature of the solute, eluent and stationary phase. The correlation between retention constants of 16,17-secoestrone derivatives obtained from reversed-phases and commercially available ACD log P software (Advanced Chemistry, Toronto, Canada) has also been examined.     

A simplified method for defining air humidification and dehumidification requirements

Nowadays, the use of ventilation systems is very common. In museums and exhibition rooms, they are used to guarantee the optimal temperature and humidity conditions for valuable and irreplaceable objects. Ventilation systems are increasingly used even in residential and office buildings. In Austria and other similar climates, winter outdoor air is very dry. Because of the constant air exchange using a ventilation system, the relative humidity of indoor air is often lower than the comfort limit, so that air humidification is necessary. In the same rooms, air must be dehumidified during the summer months. Humidification and dehumidification should be minimized as they are processes that consume a large amount of energy. The problem is that until now, it has been unclear how much humidification or dehumidification is necessary and could not be calculated easily. In this investigation, a simplified method for determining the humidification and dehumidification demands taking into account the effective moisture capacity of the room was developed. The developed model is used for the calculation of the energy balance and will be integrated into the program for energy certification in Austria.     

A simple and selective spectrophotometric flow injection method for the determination of ultra trace amounts of phenylhydrazine by its inhibition effect on the reaction of victoria blue B and bromate in micellar medium

In this work, a simple, selective and rapid flow injection method has been developed for the determination of phenylhydrazine. The method is based on its inhibition effect on the reaction of victoria blue B and bromate in acidic micellar medium. The reaction was monitored spectrophotometrically by measuring victoria blue B absorbance at λ_max = 632 nm. The reagents and manifold variables, which have influences on the sensitivity, were investigated and the optimum conditions were established. The optimized conditions made it possible to determine phenylhydrazine in the ranges of 7–370 nM with a detection limit of 5 nM and a sample rate of 25 ± 5 samples/h. The proposed method has been successfully applied to the determination of phenylhydrazine in human serum and water samples.     

Critical issues in breast imaging by vibro-acoustography

Clinically, there are two important issues in breast imaging: detection of microcalcifications and identification of mass lesions. X-ray mammography is the main imaging method used for detection of microcalcification, and ultrasound imaging is normally used for detection of mass lesions in breast. Both these methods have limitations that reduce their clinical usefulness. For this reasons, alternative breast imaging modalities are being sought. vibro-acoustography is an imaging modality that has emerged in recent years. This method is based on low-frequency harmonic vibrations induced in the object by the radiation force of ultrasound. This paper describes potential applications of vibro-acoustography for breast imaging and addresses the critical imaging issues such as detection of microcalcifications and mass lesions in breast. Recently, we have developed a vibro-acoustography system for in vivo breast imaging and have tested it on a number of volunteers. Resulting images show soft tissue structures and calcifications within breast with high contrast, high resolution, and no speckles. The results have been verified using X-ray mammography. The encouraging results from in vitro and in vivo experiments suggest that further development of vibro-acoustography technology may lead to a new clinical tool that can be used to detect microcalcifications as well as mass lesions in breast.     

Vibro-acoustography and multifrequency image compounding

Vibro-acoustography is an ultrasound based imaging modality that can visualize normal and abnormal soft tissue through mapping the acoustic response of the object to a harmonic radiation force at frequency Δf induced by focused ultrasound. In this method, the ultrasound energy is converted from high ultrasound frequencies to a low acoustic frequency (acoustic emission) that is often two orders of magnitude smaller than the ultrasound frequency. The acoustic emission is normally detected by a hydrophone. Depending on the setup, this low frequency sound may reverberate by object boundaries or other structures present in the acoustic paths before it reaches the hydrophone. This effect produces an artifact in the image in the form of gradual variations in image intensity that may compromise image quality. The use of tonebursts with finite length yields acoustic emission at Δf and at sidebands centered about Δf. Multiple images are formed by selectively applying bandpass filters on the acoustic emission at Δf and the associated sidebands. The data at these multiple frequencies are compounded through both coherent and incoherent processes to reduce the acoustic emission reverberation artifacts. Experimental results from a urethane breast phantom are described. The coherent and incoherent compounding of multifrequency data show, both qualitatively and quantitatively, the efficacy of this reverberation reduction method. This paper presents theory describing the physical origin of this artifact and use of image data created using multifrequency vibro-acoustography for reducing reverberation artifacts.     

A complete convergence theorem for stationary regularly varying multivariate time series

For a class of stationary regularly varying and weakly dependent multivariate time series (X _n ), we prove the so-called complete convergence result for the space–time point processes of the form \(N_{n} = \sum _{i=1}^{n} \delta _{(i/n, \boldsymbol {X}_{i}/a_{n})}.\) As an application of our main theorem, we give a simple proof of the invariance principle for the corresponding partial maximum process.     

Anti-Inflammatory Effect of Phytoncide in an Animal Model of Gastrointestinal Inflammation

Background: Phytoncide is known to have antimicrobial and anti-inflammatory properties. Purpose: This study was carried out to confirm the anti-inflammatory activity of two types of phytoncide extracts from pinecone waste. Methods: We made two types of animal models to evaluate the efficacy, an indomethacin-induced gastroenteritis rat model and a dextran sulfate sodium-induced colitis mouse model. Result: In the gastroenteritis experiment, the expression of induced-nitric oxide synthase (iNOS), a marker for inflammation, decreased in the phytoncide-supplemented groups, and gastric ulcer development was significantly inhibited (p < 0.05). In the colitis experiment, the shortening of the colon length and the iNOS expression were significantly suppressed in the phytoncide-supplemented group (p < 0.05). Conclusions: Through this study, we confirmed that phytoncide can directly inhibit inflammation in digestive organs. Although further research is needed, we conclude that phytoncide has potential anti-inflammatory properties in the digestive tract and can be developed as a functional agent.     

Molecular above-threshold ionization with a circularly polarized laser field

Molecular above-threshold ionization by a circularly polarized laser field is investigated. Our theoretical approach is based on the modified molecular strong-field approximation. Various gauge-dependent versions of this approximation are considered and homonuclear and heteronuclear molecular species characterized by different symmetries are used as targets. As in the case of a linearly polarized field, the imprint of the molecular multicenter structure can be observed in the above-threshold ionization spectra. It manifests itself as minima in the rate of the ionized electrons as a function of their energy. The locations of these minima are strongly influenced by the symmetry of the corresponding highest occupied molecular orbital as well as the internuclear separation. Analyzing the interference structures of the electron spectra one can obtain information about the molecular symmetry.     

Spectroscopic studies of dactinomycin and vinorelbine binding to deoxyribonucleic acid and chromatin

Dactinomycin and vinorelbine are anticancer drugs, widely used as chemotherapeutic agents for the treatment of various cancers. This study represents an attempt to compare the effect of these drugs on DNA and soluble chromatin employing UV/vis, fluorescence and circular dichroism spectroscopy techniques. The results demonstrated that the absorbance at 260 and 210 nm was decreased and quenching of drugs with DNA and chromatin chromospheres induced reduction of fluorescence emission intensity. Circular dichroism profiles showed that the binding of drugs induced structural changes in both positive and negative extremes of DNA and chromatin revealing release or displacement of histone proteins from chromatin upon dactinomycin binding whereas vinorelbine preceded the chromatin into compaction. The results suggest that dactinomycin shows higher affinity to DNA compared to chromatin, whereas, vinorelbine recognizes the chromatin structure with higher affinity than free DNA. Also chromatin proteins play a fundamental role in drug-chromatin complex process.     

Flavonoid-DNA binding studies and thermodynamic parameters

Interactional studies of new flavonoid derivatives (Fl) with chicken blood ds.DNA were investigated spectrophotometrically in DMSO-H2O (9:1 v/v) at various temperatures. Spectral parameters suggest considerable binding between the flavonoid derivatives studied and ds.DNA. The binding constant values lie in the enhanced-binding range. Thermodynamic parameters obtained from UV studies also point to strong spontaneous binding of Fl with ds.DNA. Viscometric studies complimented the UV results where a small linear increase in relative viscosity of the DNA solution was observed with added optimal flavonoid concentration. An overall mixed mode of interaction (intercalative plus groove binding) is proposed between DNA and flavonoids. Conclusively, investigated flavonoid derivatives are found to be strong DNA binders and seem to be promising drug candidates like their natural analogues.